Skip to Content

Coronavirus information for Feinberg.

Download the full-sized PDF of A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study
Download the file

Actions

Download Analytics Citations

Export to: EndNote

Collections

This file is in the following collections:

COVID-19 Community

A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study Open Access (recommended)

Coryell MP, Iakiviak M, Pereira N, Murugkar PP, Rippe J, Williams DB, Heald-Sargent T, Sanchez-Pinto LN, Chavez J, Hastie JL, Sava RL, Lien CZ, Wang TT, Muller WJ, Fischbach MA, Carlson PE. A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study. Lancet Microbe. 2021;2(6):E259-E266.

Descriptions

Resource type(s)
Article
Keyword
COVID-19
Rights
Attribution 4.0 International

Creator
Coryell, Michael P.
Iakiviak, Mikhail
Pereira, Nicole
Murugkar, Pallavi P.
Rippe, Jason
Williams, David B.
Heald-Sargent, Taylor Alis
Sanchez-Pinto, L. Nelson
Chavez, Jairo
Hastie, Jessica L.
Sava, Rosa L.
Lien, Christopher Z.
Wang, Tony T.
Muller, William J.
Fischbach, Michael A.
Carlson, Paul E., Jr.
Abstract
Background Faecal shedding of SARS-CoV-2 has raised concerns about transmission through faecal microbiota transplantation procedures. Validation parameters of authorised tests for SARS-CoV-2 RNA detection in respiratory samples are described in product labelling, whereas the published methods for SARS-CoV-2 detection from faecal samples have not permitted a robust description of the assay parameters. We aimed to develop and validate a test specifically for detection of SARS-CoV-2 in human stool. Methods In this validation study, we evaluated performance characteristics of a reverse transcriptase real-time PCR (RT-rtPCR) test for detection of SARS-CoV-2 in human stool specimens by spiking stool with inactivated SARS-CoV-2 material. A modified version of the US Centers for Disease Control and Prevention RT-rtPCR SARS-CoV-2 test was used for detection of viral RNA. Analytical sensitivity was evaluated in freshly spiked stool by testing two-fold dilutions in replicates of 20. Masked samples were tested by a second laboratory to evaluate interlaboratory reproducibility. Short-term (7-day) stability of viral RNA in stool samples was assessed with four different stool storage buffers (phosphate-buffered saline, Cary-Blair medium, Stool Transport and Recovery [STAR] buffer, and DNA/RNA Shield) kept at -80 degrees C, 4 degrees C, and ambient temperature (approximately 21 degrees C). We also tested clinical stool and anal swab specimens from patients who were SARS-CoV-2 positive by nasopharyngeal testing. Findings The lower limit of detection of the assay was found to be 3000 viral RNA copies per g of original stool sample, with 100% detection across 20 replicates assessed at this concentration. Analytical sensitivity was diminished by approximately two times after a single freeze-thaw cycle at -80 degrees C. At 100 times the limit of detection, spiked samples were generally stable in all four stool storage buffers tested for up to 7 days, with maximum changes in mean threshold cycle values observed at -80 degrees C storage in Cary-Blair medium (from 29.4 [SD 0.27] at baseline to 30.8 [0.17] at day 7; p<0.0001), at 4 degrees C storage in DNA/RNA Shield (from 28.5 [0.15] to 29.8 [0.09]; p=0.0019), and at ambient temperature in STAR buffer (from 30.4 [0.24] to 32.4 [0.62]; p=0.0083). 30 contrived SARS-CoV-2 samples were tested by a second laboratory and were correctly identified as positive or negative in at least one of two rounds of testing. Additionally, SARS-CoV-2 RNA was detected using this assay in the stool and anal swab specimens of 11 of 23 individuals known to be positive for SARS-CoV-2. Interpretation This is a sensitive and reproducible assay for detection of SARS-CoV-2 RNA in human stool, with potential uses in faecal microbiota transplantation donor screening, sewage monitoring, and further research into the effects of faecal shedding on the epidemiology of the COVID-19 pandemic. Copyright Published by Elsevier Ltd.
Related URL
Publisher
ELSEVIER
Date Created
2021-06
Original Identifier
(PMID) 33821247
Grants and funding
National Institute of Allergy and Infectious Diseases, US National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID); Center for Biologics Evaluation and Research, US Food and Drug Administration
DOI
10.1016/S2666-5247(21)00059-8

File Details

File Properties
Mime type: application/pdf
File size: 520.9 kB